ABSTRACT
BACKGROUND:Adipose-derived stem cels have similar characteristics of bone marrow mesenchymal stem cels, including adhesion and fibroblast-like clone formation. In addition, adipose-derived stem cels can differentiate into bone, adipose, cartilage. OBJECTIVE:To compare the biological characteristics of C57 mouse adipose-derived and bone marrow mesenchymal stem cels. METHODS:Mesenchymal stem cels were obtained respectively from the adipose and bone marrow of C57 mice under sterile condition. Adipose-derived and bone marrow mesenchymal stem cels were isolated and purified in vitro to determine cellmorphology, suface marker, growth kinetics, differentiation potential and Notch signal related gene. RESULTS AND CONCLUSION:Morphology was similar between adipose-derived and bone marrow mesenchymal stem cels under an optical microscope. Adipose-derived and bone marrow mesenchymal stem cels at passage 3 expressed CD29, CD105, Sca-1, but not expressed CD34, CD133. In addition, bone marrow mesenchymal stem cels also expressed CD45. The growth kinetics and cellclone analysis indicated that the proliferation rate of adipose-derived mesenchymal stem cels was significantly faster than that of bone marrow mesenchymal stem cels. Both of adipose-derived and bone marrow mesenchymal stem cels were induced to osteoblasts, adipocytes and chondrocytes, but the adipose-derived mesenchymal stem cels were easier to the osteogenetic differentiation than bone marrow mesenchymal stem cels. Notch signal related gene detection shown that adipose-derived mesenchymal stem cels expressed lower levels of Jagged-1 mRNA, but higher levels of Hes-1 mRNA compared with bone marrow mesenchymal stem cels. These findings suggest that the proliferation rate of adipose-derived mesenchymal stem cels is significantly faster than that of bone marrow mesenchymal stem cels, and the former is apt to the osteogenetic differentiation and may be related with the expressed levels of Hes-1 mRNA.
ABSTRACT
BACKGROUND:Under mitomycin C treatment, feeder cells appear to have restricted proliferation, but they are stil able to secret different cytokines. Non-mesenchymal stem cells from the bone marrow and secreted factors in plasma maintain the micro-environment suitable for the growth of mesenchymal stem cells that can improve the yield of mesenchymal stem cells. OBJECTIVE:To study the biological characteristics of C57 mouse bone marrow mesenchymal stem cells isolated using a whole bone marrow adherent culture technique. METHODS:Using the whole bone marrow adherent culture technique, purified and amplified C57 mouse bone marrow mesenchymal stem cells were harvested. cellproliferation kinetics, immune cellsurface markers, multiple differentiation potential and cellcycle were detected. RESULTS AND CONCLUSION:Using the whole bone marrow culture, mouse bone marrow mesenchymal stem cells were harvested and capable of adhering to the plastic culture vessel. The obtained cells expressed CD45, CD105 and Sca-1, but were negative for CD34, CD33 and C-kit. The doubling time was (57.11±1.5) hours. The cells could be induced to differentiate into osteoblasts, adipocytes and chondrocytes. The cellcycle analysis showed that 64%of cells were in G 0-G 1 phase. These indicates that C57 mouse bone marrow mesenchymal stem cells isolated using a whole bone marrow adherent culture technique have biological characteristics of mesenchymal stem cells.